Human CD25/IL-2R alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10201
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2604B
Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived human CD25/IL-2R alpha
Glu22-Cys213
Accession # P01589
Glu22-Cys213
Accession # P01589
Specificity
Detects human CD25/IL-2R alpha in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CD25/IL-2R alpha Antibody
Detection of Human CD25/IL-2R alpha by Western Blot.
Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB10201) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human CD25/IL-2R alpha Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HDLM‑2 human Hodgkin's lymphoma cell line
Sample: HDLM‑2 human Hodgkin's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Entrez Gene IDs
Gene Symbol
IL2RA
UniProt
Additional CD25/IL-2R alpha Products
Product Documents for Human CD25/IL-2R alpha Antibody
Product Specific Notices for Human CD25/IL-2R alpha Antibody
For research use only
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