Human CD28 Antibody

Name: Human CD28 Antibody
Brand: R&D Systems
SKU: MAB11412

R&D Systems, part of Bio-Techne | Catalog # MAB11412

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

Key Product Details

Species Reactivity:	Human
Applications:	Functional Assay
Label:	Unconjugated
Specificity:	Detects human CD28 in direct ELISAs.

View Full Technical Details for Human CD28 Antibody

Technical Data
Citations / Reviews

Applications for Human CD28 Antibody

	Recommended Concentration	Sample
T Cell Stimulation	3 µg/mL	Human PBMCs

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD28 Antibody

	T Cell Stimulation Recombinant Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 recombinant antibody enhances IL-2 secretion in Jurkat cells (immortalized human T cell line) treated with 5 ng/mL phorbol myristate acetate (PMA) and 0.9 µM calcium ionomycin. Antibody-mediated stimulation occurred in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA kit. The ED₅₀ for this effect is 5-50 ng/mL.

Formulation, Preparation and Storage

Reconstitution	Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD28

The Bio-Techne CD28 GMP antibody is derived from the 15e8 clone. CD28 is a critical protein expressed on the surface of T cells that provides a co-stimulatory signal necessary for T cell activation and proliferation. CD28 is structurally similar to CTLA-4, with both molecules exhibiting structural homology to the immunoglobulin (Ig) gene superfamily. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain, and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2.

Item	Value
Entrez Gene IDs	940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)
Alternate Names	CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

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Human CD28 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11412

Key Product Details

Applications for Human CD28 Antibody

Scientific Data Examples for Human CD28 Antibody

T Cell Stimulation

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD28

Customer Reviews for Human CD28 Antibody

Product Documents for Human CD28 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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