Human CD30/TNFRSF8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2291
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 81316
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD30/TNFRSF8
Phe19-Lys379
Accession # P28908
Phe19-Lys379
Accession # P28908
Specificity
Detects human CD30/TNFRSF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, recombinant mouse CD30, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhHVEM, rhLT beta R, rhNGF R, rhOPG, rhRANK, rhTNF RI, or rhTNF RII is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD30/TNFRSF8 Antibody
Human CD30/TNFRSF8 ELISA Standard Curve.
Recombinant Human CD30/TNFRSF8 protein was serially diluted 2-fold and captured by Mouse Anti-Human CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB2291) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (AF229) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Applications for Human CD30/TNFRSF8 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF229).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CD30/TNFRSF8 DuoSet ELISA Kit (Catalog # DY6126-05) for convenient development of a sandwich ELISA.
Western Blot
1 µg/mL
Sample: Recombinant Human CD30/TNFRSF8 Fc Chimera (Catalog # 813-CD) under non-reducing conditions only
Sample: Recombinant Human CD30/TNFRSF8 Fc Chimera (Catalog # 813-CD) under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD30/TNFRSF8
References
- Kennedy, M.K. et al. (2006) Immunology 118:143.
- Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.
- Durkop, H. et al. (1992) Cell 68:421.
- Hamann, D. et al. (1996) J. Immunol. 156:1387.
- Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147.
- Gruss, H.-J. et al. (1994) Blood 83:2045.
- Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174.
- Del Prete, G. et al. (1995) J. Exp. Med. 182:1655.
- Harlin, H. et al. (2002) J. Immunol. 169:2451.
- Amakawa, R. et al. (1996) Cell 84:551.
- Chiarle, R. et al. (1999) J. Immunol. 163:194.
- Vinante, F. et al. (2002) Blood 99:52.
- Hansen, H.P. et al. (1995) Int. J. Cancer 63:750.
- Hansen, H.P. et al. (2000) J. Immunol. 165:6703.
- Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.
Alternate Names
CD30, TNFRSF8
Gene Symbol
TNFRSF8
UniProt
Additional CD30/TNFRSF8 Products
Product Documents for Human CD30/TNFRSF8 Antibody
Product Specific Notices for Human CD30/TNFRSF8 Antibody
For research use only
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