Human CD31/PECAM-1 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3567P
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Knockout Validated
Cited:
Bioassay, Cell Selection, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 9G11
Product Specifications
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD31/PECAM-1 PE-conjugated Antibody
Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD31/PECAM-1 in THP-1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was performed using our Membrane-Associated Staining protocol.
CD31/PECAM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.
CD31/PECAM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). No staining in the CD31/PECAM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human CD31/PECAM-1 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood granulocytes and THP-1 human acute monocytic leukemia cell line
Sample: Human peripheral blood granulocytes and THP-1 human acute monocytic leukemia cell line
Knockout Validated
CD31/PECAM-1 is specifically detected in THP-1 myelogenous leukemia parental cell line but is not detectable in CD31/PECAM-1 knockout THP-1 cell line.
Reviewed Applications
Read 2 reviews rated 4 using FAB3567P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD31/PECAM-1
The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alphav beta3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Alternate Names
CD31, EndoCAM, PECA1, PECAM-1, PECAM1
Gene Symbol
PECAM1
Additional CD31/PECAM-1 Products
Product Documents for Human CD31/PECAM-1 PE-conjugated Antibody
Product Specific Notices for Human CD31/PECAM-1 PE-conjugated Antibody
For research use only
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