Human CD34 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7227
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # QBEnd10
Product Specifications
Immunogen
Human endothelial vesicles
Specificity
Detects human CD34 in direct ELISAs
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD34 Antibody
Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry.
KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB7227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD34 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: KG‑1a human acute myelogenous leukemia cell line
Sample: KG‑1a human acute myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB7227 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD34
Alternate Names
CD34, HPCA1
Gene Symbol
CD34
Additional CD34 Products
Product Documents for Human CD34 Antibody
Product Specific Notices for Human CD34 Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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