Detects human CD35 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat CSMD1, recombinant human (rh) CSMD2, rhCSMD3, rhHABP1/C1QBP, or rhComplement Factor H is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human CD35 Antibody

Detection of CD35 in Human PBMCs by Flow Cytometry.

Human peripheral blood lymphocytes (PBMC) were stained with Human CD35 Monoclonal Antibody (Catalog # MAB5748) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB002 and IC002P, respectively).

Applications for Human CD35 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes (PBMC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD35

CD35, also known as complement receptor 1 (CR1), is a 220-300 kDa N-glycosylated member of the RCA (regulators of complement activation) family of proteins. CD35 binds and internalizes particles and immune complexes that are opsonized with MBL or complement components C3b, C3i, C4b, or C1q. CD35 additionally protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases. The extracellular domain (ECD) of human CD35 contains 30 tandem SCR/SUSHI repeats. A soluble form of the CD35 ECD circulates in the serum. A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35. Cell surface CD35 is widely expressed on hematopoietic cells.

Alternate Names

C3BR, CD35, CR1, KN

Entrez Gene IDs

1378 (Human)

Gene Symbol

CR1

UniProt

CAA68755

Additional CD35 Products

Product Documents for Human CD35 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD35 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD35 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5748

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity