Human CD3 epsilon F(ab')2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB100R-FAB2
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # UCHT1R-FAB2-TEV/His
Product Specifications
Immunogen
Human thymocytes
Specificity
Detects human CD3 epsilon
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD3 epsilon F(ab')2 Antibody
Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry with anti-Mouse Secondary Antibody.
Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Staining was performed using our staining membrane proteins protocol.
Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry with anti-His antibody.
Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by PE-conjugated Anti-His Antibody (IC050P) and Mouse anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Staining was performed using our staining membrane proteins protocol.Applications for Human CD3 epsilon F(ab')2 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: PBMC lymphocytes
Sample: PBMC lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3 epsilon
CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
References
- Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for Human CD3 epsilon F(ab')2 Antibody
Product Specific Notices for Human CD3 epsilon F(ab')2 Antibody
For research use only
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