Human CD4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11560
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Multiplex Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2992A
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human CD4
Lys26-Trp390
Accession # P01730
Lys26-Trp390
Accession # P01730
Specificity
Detects recombinant human CD4 in Direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CD4 Antibody
Detection of CD4 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™
CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11560) at 20µg/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Detection of CD4 in Human Tonsil.
CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11560) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of CD4 in hPBMC cells (Positive) and MCF-7 cells (Negative).
CD4 was detected in fixed hPBMC cells (Positive) and absent in MCF-7 human breast cancer cell line (Negative) using Rabbit Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11560) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CD4 Antibody
Application
Recommended Usage
Immunocytochemistry
1-25 µg/mL
Sample: fixed hPBMC cells (Positive) and absent in MCF-7 human breast cancer cell line (Negative)
Sample: fixed hPBMC cells (Positive) and absent in MCF-7 human breast cancer cell line (Negative)
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Multiplex Immunofluorescence
20 µg/mL
Sample: Immersion fixed paraffin embedded sections of human tonsil
Sample: Immersion fixed paraffin embedded sections of human tonsil
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD4
CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
References
- Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
Alternate Names
CD4
Entrez Gene IDs
Gene Symbol
CD4
UniProt
Additional CD4 Products
Product Documents for Human CD4 Antibody
Product Specific Notices for Human CD4 Antibody
For research use only
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