Human CD4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11530

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB11530-100
MAB11530-SP
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Key Product Details

Species Reactivity

Human

Applications

Cell depletion

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG2 Clone # 30345-1

View all CD4 Antibodies »

Product Specifications

Immunogen

PHA-stimulated PBMCs

Specificity

Detects recombinant human CD4 in direct ELISA

Clonality

Monoclonal

Host

Human

Isotype

IgG2

Scientific Data Images for Human CD4 Antibody

Immobilized recombinant CD4 antibody supports the adhesion of SUP-T1 cells to cell culture plates.

The CD4 antibody was coated onto cell culture plates at increasing amounts. The wells were then incubated with calcein labeled SUP-T1 cells, and cell binding was detected using a fluorescence-based plate reader. The ED50 for this effect is 0.200-2.00 μg/mL.

Applications for Human CD4 Antibody

Application
Recommended Usage

Cell depletion

2 µg/mL
Sample: Selects CD4+ cells when immobilized to cell culture plate or bead
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified from cell culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
*Small pack size (-SP) is shipped at ambient temperature or with polar packs. Upon receipt, store it immediately at the temperature

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

This Bio-Techne CD4 Antibody is a reformatted version of the RPA-T4 clone.  CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

Additional CD4 Products

Product Documents for Human CD4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD4 Antibody

For research use only

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