Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9889

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry

Cited:

Immunoprecipitation, Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu116

View all CD52 Antibodies »

Product Specifications

Immunogen

Human CD52

Specificity

Detects human CD52 based on Alemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Alemtuzumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

Detection of CD52 antibody in human PBMC lymphocytes antibody by Flow Cytometry.

Detection of CD52 in human PBMC lymphocytes by Flow Cytometry.

Human PBMC lymphocytes were stained with Human Anti-Human CD52 (Alemtuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9889, filled histogram) or no primary antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD52

Alemtuzumab is a biosimilar directed against the extracellular domain of CD52. The biotinylated antibody (catalog number FAB9889B) can be used in flow cytometry or various assay format to measure CD52 expression and can be detected by conjugated streptavidin.

Alternate Names

CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP

Entrez Gene IDs

1043 (Human); 23833 (Mouse)

Gene Symbol

CD52

Additional CD52 Products

Product Documents for Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

For research use only

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