Human IgG APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # F0135
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Human IgG
Specificity
This antibody reacts with heavy chains on human IgG and with light chains on all human immunoglobulins. No reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins by IEP.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IgG APC-conjugated Antibody
Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Cetuximab) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog #F0135). View our protocol for Staining Membrane-associated Proteins.Applications for Human IgG APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent
assays, such as flow cytometry, where the primary antibody does not have
a fluorescent reporter, is of human origin, and is of IgG class. The
recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using F0135 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Human IgG APC-conjugated Antibody
Product Specific Notices for Human IgG APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...