Human CD69 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2359
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108
Gly64-Lys199
Accession # Q07108
Specificity
Detects human CD69 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse CD69 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CD69 Antibody
CD69 in Human PBMCs.
CD69 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2359) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CD69 in Human Tonsil.
CD69 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Goat Anti-Human CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2359) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CD69 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with PHA
Sample: Human peripheral blood mononuclear cells treated with PHA
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil
Western Blot
0.1 µg/mL
Sample: Recombinant Human CD69
Sample: Recombinant Human CD69
Reviewed Applications
Read 1 review rated 4 using AF2359 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD69
Alternate Names
CD69, EA-1, Leu23, MLR-3, p60
Gene Symbol
CD69
UniProt
Additional CD69 Products
Product Documents for Human CD69 Antibody
Product Specific Notices for Human CD69 Antibody
For research use only
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