Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB23591P) or isotype control antibody (IC003P, data not shown). View our protocol for Staining Membrane-associated Proteins.

Detection of CD69 in stimulated vs. naive PBMCs by Flow Cytometry.

PBMCs either treated with 5 ug/mL PHA for 2 days (A) or (B) left untreated (naive) were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB23591P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD69 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes; PBMCs treated with 5 ug/mL PHA for 2 days or left untreated

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Gene Symbol

CD69

UniProt

Q07108

Additional CD69 Products

Product Documents for Human CD69 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD69 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CD69 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB23591P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD69 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD69 PE-conjugated Antibody

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry.