Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CD74 Monoclonal Antibody (Catalog # MAB35901) or (B) Mouse IgG1 isotype control (MAB002) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Detection of CD74 in K562 Human Cell Line by Flow Cytometry.

K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human CD74 Monoclonal Antibody (Catalog # MAB35901, filled histogram) or Mouse IgG1 isotype control (MAB002,open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human CD74 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human PBMC lymphocytes and K562 myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD74

CD74 is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. The cytoplasmic portion of CD74 can be released by intramembrane cleavage and activate NF kappaB. CD74 is up-regulated in several cancers and is also expressed by non-immune cells during inflammation. This antibody was raised to a splice variant of human CD74 which lacks the Thyroglobulin type 1 repeat in the extracellular domain. Within comparable regions, human CD74 shares 70% and 73% amino acid sequence identity with mouse and rat CD74, respectively.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Entrez Gene IDs

972 (Human); 16149 (Mouse); 25599 (Rat)

Gene Symbol

CD74

UniProt

NP_004346

Additional CD74 Products

Product Documents for Human CD74 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD74 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD74 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB35901

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD74 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD74 Antibody

Detection of CD74 in Human PBMC by Flow Cytometry.