Human CD8 Antibody

Name: Human CD8 Antibody
Brand: R&D Systems
SKU: MAB11531

R&D Systems, part of Bio-Techne | Catalog # MAB11531

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Product Datasheet / COA

Recombinant Monoclonal Antibody.

Key Product Details

Species Reactivity:	Human
Immunogen:	Alpha chain of CD8 Accession # P01732
Applications:	Cell Depletion
Label:	Unconjugated
Source:	Recombinant Monoclonal Human IgG₁ Clone # 30798-1
Specificity:	Detects recombinant human CD8 in direct ELISA

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Technical Data
Citations / Reviews

Applications for Human CD8 Antibody

	Recommended Concentration	Sample
Cell depletion	2 µg/mL	Selects CD8+ cells when immobilized to cell culture plate or bead

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD8 Antibody

	Cell Depletion Immobilized recombinant CD8 antibody supports the adhesion of SUP-T1 cells to cell culture plates. The CD8 antibody was coated onto cell culture plates at increasing amounts. The wells were then incubated with calcein labeled SUP-T1 cells, and cell binding was detected using a fluorescence-based plate reader. The ED₅₀ for this effect is 0.200‑2.00 μg/mL.

Formulation, Preparation and Storage

Purification	Protein G purified from cell culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (-SP) is shipped at ambient temperature or with polar packs. Upon receipt, store it immediately at the temperature
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

This Bio-Techne CD8 antibody is a reformatted version of the RPA-T8 clone. CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. There are two isoforms of the protein, alpha and beta, each encoded by a different gene. In humans, both genes are located on chromosome 2 in position 2p12. The most common form of CD8 is composed of a CD8-alpha and CD8-beta chain, both members of the immunoglobulin superfamily with an immunoglobulin variable (IgV)-like extracellular domain connected to the membrane by a thin stalk, and an intracellular tail. Less-common homodimers of the CD8-alpha chain are also expressed on some cells.

Item	Value
Entrez Gene IDs	925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names	CD8, CD8 antigen, alpha polypeptide (p32), CD8A, CD8a molecule, CD_antigen: CD8a, LEU2, Leu2 T-lymphocyte antigen, MAL, OKT8 T-cell antigen, T cell co-receptor, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T-cell antigen, p32

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Product Documents for Human CD8 Antibody

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