Human CD8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11531

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB11531-100
MAB11531-SP
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Key Product Details

Species Reactivity

Human

Applications

Cell depletion

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # 30798-1

View all CD8 Antibodies »

Product Specifications

Immunogen

Alpha chain of CD8
Accession # P01732

Specificity

Detects recombinant human CD8 in direct ELISA

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for Human CD8 Antibody

Immobilized recombinant CD8 antibody supports the adhesion of SUP-T1 cells to cell culture plates.

The CD8 antibody was coated onto cell culture plates at increasing amounts. The wells were then incubated with calcein labeled SUP-T1 cells, and cell binding was detected using a fluorescence-based plate reader. The ED50 for this effect is 0.200-2.00 μg/mL.

Applications for Human CD8 Antibody

Application
Recommended Usage

Cell depletion

2 µg/mL
Sample: Selects CD8+ cells when immobilized to cell culture plate or bead

Formulation, Preparation, and Storage

Purification

Protein G purified from cell culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
*Small pack size (-SP) is shipped at ambient temperature or with polar packs. Upon receipt, store it immediately at the temperature

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

This Bio-Techne CD8 antibody is a reformatted version of the RPA-T8 clone.  CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. There are two isoforms of the protein, alpha and beta, each encoded by a different gene. In humans, both genes are located on chromosome 2 in position 2p12. The most common form of CD8 is composed of a CD8-alpha and CD8-beta chain, both members of the immunoglobulin superfamily with an immunoglobulin variable (IgV)-like extracellular domain connected to the membrane by a thin stalk, and an intracellular tail. Less-common homodimers of the CD8-alpha chain are also expressed on some cells.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P01732 (Human)

Additional CD8 Products

Product Documents for Human CD8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD8 Antibody

For research use only

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