Human CD82/Kai-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4616
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Array Development, Cell-based ELISA, Microarray
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 423524
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CD82/Kai‑1
Met1-Tyr267
Accession # AAH01821
Met1-Tyr267
Accession # AAH01821
Specificity
Detects human CD82/Kai‑1. Stains human CD82/Kai‑1 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD82/Kai-1 Antibody
Detection of CD82/Kai-1 in Human Granulocytes by Flow Cytometry.
Human whole blood granulocytes were stained with Mouse Anti-Human CD82/Kai-1 Monoclonal Antibody (Catalog # MAB4616, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).Applications for Human CD82/Kai-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood granulocytes
Sample: Human whole blood granulocytes
Reviewed Applications
Read 4 reviews rated 4.3 using MAB4616 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD82/Kai-1
CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.
Alternate Names
CD82, IA4, KAI1, SAR2, ST6, TSPAN27
Gene Symbol
CD82
UniProt
Additional CD82/Kai-1 Products
Product Documents for Human CD82/Kai-1 Antibody
Product Specific Notices for Human CD82/Kai-1 Antibody
For research use only
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