Human CD9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1880
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Cell Purification, Cell Selection, ELISA Capture, ELISA Detection, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 209306
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CD9
Met1-Val228
Accession # P21926
Met1-Val228
Accession # P21926
Specificity
Detects human CD9. Stains human CD9 transfectants, but not irrelevant transfectants in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD9 Antibody
Detection of CD9 in Human Platelets by Flow Cytometry.
Human peripheral blood platelets were stained with Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB1880, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Detection of Human CD9 by Western Blot
Validation of protein expression by Western blotting.All four exosome samples and their corresponding cell lines were used for validation. Furthermore, supernatant from the pelleted exosomes was used as a control. The selected proteins FASN, XPO1, CD9 and PDCD6IP, were tested with ENO1 and GAPDH as controls. PSA was tested to confirm it is secreted through alternative secretion pathway and therefore not present within exosomes. The nearest protein marker (kDa) is indicated for each blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24391718), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human CD9 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human platelets
Sample: Human platelets
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD9
CD9, also known as motility-related protein-1 (MRP-1), is a tetraspan family glycoprotein expressed by a variety of hematopoietic and epithelial cells. It forms homotypic associations as well as heterotypic associations with other tetraspan proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation.
Alternate Names
CD9, DRAP-27, MIC3, p24, TSPAN29
Gene Symbol
CD9
UniProt
Additional CD9 Products
Product Documents for Human CD9 Antibody
Product Specific Notices for Human CD9 Antibody
For research use only
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