Human CDCP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2666
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
Phe30-Leu666
Accession # NP_073753
Phe30-Leu666
Accession # NP_073753
Specificity
Detects human CDCP1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CDCP1 Antibody
CDCP1 in Human Colon Cancer Tissue.
CDCP1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2666) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the apices of colonic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CDCP1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: SW480 human colorectal adenocarcinoma cell line
Sample: SW480 human colorectal adenocarcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human CDCP1
Sample: Recombinant Human CDCP1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDCP1
Long Name
CUB Domain Containing Protein 1
Alternate Names
CD318, SIMA135
Gene Symbol
CDCP1
UniProt
Additional CDCP1 Products
Product Documents for Human CDCP1 Antibody
Product Specific Notices for Human CDCP1 Antibody
For research use only
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