Human CEACAM-8/CD66b Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4246
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CEACAM‑8/CD66b
Gln35-His141
Accession # P31997
Gln35-His141
Accession # P31997
Specificity
Detects human CEACAM‑8/CD66b in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) CEACAM-3, rhCEACAM-6, and rhCEACAM-7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CEACAM-8/CD66b Antibody
Detection of CEACAM-8/CD66b in Human Granulocytes by Flow Cytometry.
Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM-8/CD66b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4246, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).Applications for Human CEACAM-8/CD66b Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood granulocytes
Sample: Human whole blood granulocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human CEACAM-8/CD66b
Sample: Recombinant Human CEACAM-8/CD66b
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEACAM-8/CD66b
CEACAM-8 (carcinoembryonic antigen-related cell adhesion molecule 8; also CD66b, CD67 and NCA-95) is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
Alternate Names
CD66b, CD67, CEACAM8, CGM6, NCA-95
Gene Symbol
CEACAM8
UniProt
Additional CEACAM-8/CD66b Products
Product Documents for Human CEACAM-8/CD66b Antibody
Product Specific Notices for Human CEACAM-8/CD66b Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...