Human CEBP epsilon Antibody

Name: Human CEBP epsilon Antibody
Brand: R&D Systems
SKU: MAB6726

R&D Systems, part of Bio-Techne | Catalog # MAB6726

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 695345

View all CEBP epsilon Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEBP epsilon
Thr151-Arg210
Accession # Q15744

Specificity

Detects human CEBP epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEBP alpha, beta, delta, gamma, or zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human CEBP epsilon Antibody

Detection of Human CEBP epsilon by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human CEBP e Monoclonal Antibody (Catalog # MAB6726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP e at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

CEBP epsilon in HeLa Human Cell Line.

CEBP e was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human CEBP e Monoclonal Antibody (Catalog # MAB6726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CEBP epsilon Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEBP epsilon

CCAAT-enhancer binding protein epsilon (CEBP epsilon) is a 32 kDa basic-leucine zipper transcription factor that is expressed in myeloblasts, granulocytes, and eosinophils. Multiple isoforms of human CEBP epsilon are generated by alternate splicing and alternative translation initiation sites. All four isoforms contain the bZIP domain (aa 208-267) but exert different effects on myeloid differentiation. Full length CEBP epsilon and the 30 kDa isoform (aa 33-281) cooperate with c-Myb to activate the transcription of genes involved in myeloid differentiation. The 27 kDa isoform (which lacks aa 69-97) and the 14 kDa isoform (which begins at Met153) function as transcriptional repressors in eosinophils. Within aa 151-210, human CEBP epsilon shares 93% amino acid sequence identity with mouse and rat CEBP epsilon, respectively.

Long Name

CCAAT/enhancer-Binding Protein epsilon

Alternate Names

CEBPE

Entrez Gene IDs

1053 (Human); 110794 (Mouse); 25410 (Rat)

Gene Symbol