SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human CELSR3 PE-conjugated Monoclonal Antibody (Catalog # FAB7278P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CELSR3 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: SH‑SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CELSR3

CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 98% aa sequence identity with mouse CELSR3.

Long Name

Cadherin, EGF LAG Seven-pass G-type Receptor 3

Alternate Names

CDHF11, EGFL1, HFMI1, MEGF2, RESDA1

Entrez Gene IDs

1951 (Human); 107934 (Mouse); 83466 (Rat)

Gene Symbol

CELSR3

UniProt

Q9NYQ7 (Human)

Additional CELSR3 Products

Product Documents for Human CELSR3 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CELSR3 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CELSR3 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7278P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CELSR3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CELSR3 PE-conjugated Antibody

Detection of CELSR3 in SH-SY5Y Human Cell Line by Flow Cytometry.