Human CGRP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB83503
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2413G
Product Specifications
Immunogen
Human CGRP synthetic peptide (Tocris Catalog # 3012)
Accession # P06881-1
Accession # P06881-1
Specificity
Detects human CGRP in direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CGRP Antibody
CGRP in Human Dorsal Root Ganglion.
CGRP was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglion using Rabbit Anti-Human CGRP Monoclonal Antibody (Catalog # MAB83503) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human CGRP by Western Blot.
Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CGRP Monoclonal Antibody (Catalog # MAB83503) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CGRP at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human CGRP by Simple WesternTM.
Simple Western lane view shows lysates of TT human medullary thyroid cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for CGRP2 at approximately 3 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human CGRP Monoclonal Antibody (Catalog # MAB83503) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.Applications for Human CGRP Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human dorsal root ganglion
Sample: Immersion fixed paraffin-embedded sections of human dorsal root ganglion
Simple Western
10 µg/mL
Sample: TT human medullary thyroid cancer cells
Sample: TT human medullary thyroid cancer cells
Western Blot
0.5 µg/mL
Sample: TT human medullary thyroid cancer cell line
Sample: TT human medullary thyroid cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CGRP1
Long Name
Calcitonin Gene Related Peptide I
Alternate Names
a-CGRP, alpha-CGRP, CGRP 1, CGRP alpha, CGRP I
Entrez Gene IDs
796 (Human)
Gene Symbol
CALCA
UniProt
Additional CGRP1 Products
Product Documents for Human CGRP Antibody
Product Specific Notices for Human CGRP Antibody
For research use only
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