Human CHIP/STUB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4685
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CHIP/STUB1
Met1-Tyr303
Accession # Q9UNE7
Met1-Tyr303
Accession # Q9UNE7
Specificity
Detects human CHIP/STUB1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CHIP/STUB1 Antibody
Detection of Human CHIP/STUB1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CHIP/STUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4685) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CHIP/STUB1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
CHIP/STUB1 in HeLa Human Cell Line.
CHIP/STUB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human CHIP/STUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4685) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human CHIP/STUB1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CHIP/STUB1
STUB1 (STIP1 homology and U-box-containing protein 1, also known as C-terminus of Hsc70-interacting protein, CHIP) is a 35 kDa ubiquitin ligase that participates in intracellular protein folding/refolding and degradation. It is highly expressed in striated muscle. STUB1/CHIP may complex with the molecular chaperones Hsp70 and Hsp90, or act by itself. In either event, it regulates protein stability and ubiquitination. Human STUB1/CHIP is 303 amino acids (aa) in length. It contains one TPR domain (aa 27‑127) plus a C-terminal U-Box (aa 226‑299). Its TRP domain interacts with heat shock proteins, while the U-Box participates in ubiquitination. There is one isoform that shows an alternate start site at Met73. Human and mouse STUB1 are 97% aa identical.
Long Name
C-terminus of Hsc70 Interacting Protein
Alternate Names
HSPABP2, NY-CO-7, SDCCAG7, STUB1, UBOX1
Gene Symbol
STUB1
UniProt
Additional CHIP/STUB1 Products
Product Documents for Human CHIP/STUB1 Antibody
Product Specific Notices for Human CHIP/STUB1 Antibody
For research use only
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