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Human Choline Acetyltransferase/ChAT Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3447

R&D Systems, part of Bio-Techne
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BAF3447

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Choline Acetyltransferase/ChAT Isoform R
Ala2-Pro630
Accession # NP_066266

Specificity

Detects human Choline Acetyltransferase/ChAT in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Choline Acetyltransferase/ChAT Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Choline Acetyltransferase/ChAT Isoform R
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Choline Acetyltransferase/ChAT

ChAT catalyzes the production of the neurotransmitter acetylcholine (Ach), which is required for cholinergic neuron communication. It serves as a marker for functional cholinergic neurons in the central and peripheral nervous systems. At least six ChAT mRNAs encoding 69 kDa, 82 kDa and 74 kDa ChAT proteins have been identified. Compared to the 82 kDa form, 69 kDa ChAT lacks the N-terminal 118 amino acid extension containing a nuclear localization signal. As a result the 69 kDa ChAT is primarily localized to the cytoplasm. Human 69 kDa ChAT shares 86% amino acid sequence identity with the mouse or rat ChAT.

Alternate Names

CHAT, Choline acetylase

Entrez Gene IDs

1103 (Human); 12647 (Mouse); 290567 (Rat)

Gene Symbol

CHAT

UniProt

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Human Choline Acetyltransferase/ChAT Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Choline Acetyltransferase/ChAT Biotinylated Antibody

For research use only

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