Human Chondroadherin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8218
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant Chondroadherin
Cys23-His359
Accession # O15335
Cys23-His359
Accession # O15335
Specificity
Detects human Chondroadherin in direct ELISAs and Western Blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Chondroadherin Antibody
Detection of Human Chondroadherin by Western Blot.
Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Chondroadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8218) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chondroadherin at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chondroadherin in Human Cartilage.
Chondroadherin was detected in immersion fixed paraffin-embedded sections of human cartilage using Sheep Anti-Human Chondroadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8218) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Chondroadherin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cartilage
Sample: Immersion fixed paraffin-embedded sections of human cartilage
Western Blot
2 µg/mL
Sample: Human cartilage tissue
Sample: Human cartilage tissue
Reviewed Applications
Read 1 review rated 4 using AF8218 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chondroadherin
Chondroadherin is an approximately 36 kDa secreted glycosaminoglycan that contains nine tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains. It is a component of the extracellular matrix in cartilage, tendon, bony growth plates, skeletal and cardiac muscle, lung, gastrointestinal tract, pancreas, cerebellum, and eye. It is cleaved by HTRA1/Omi during intervertebral disc degeneration. Chondroadherin functions as an adhesion protein by binding to Integrin a2/b1, Collagen II, and Collagen VI. It also promotes complement activation through binding to C1q and Complement Factor H. Mature human Chondroadherin shares 94% amino acid sequence identity with mouse and rat Chondroadherin.
Alternate Names
CHAD, SLRR4A
Gene Symbol
CHAD
UniProt
Additional Chondroadherin Products
Product Documents for Human Chondroadherin Antibody
Product Specific Notices for Human Chondroadherin Antibody
For research use only
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