Human Chondrolectin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2576
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 396305
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chondrolectin
Arg22-Asn216
Accession # Q9H9P2
Arg22-Asn216
Accession # Q9H9P2
Specificity
Detects human Chondrolectin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Chondrolectin Antibody
Detection of Chondrolectin in PC-3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
Chondrolectin in PC‑3 Human Cell Line.
Chondrolectin was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Chondrolectin Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: PC-3 human prostate cancer cell line
Sample: PC-3 human prostate cancer cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line
Sample: Immersion fixed PC-3 human prostate cancer cell line
Western Blot
1 µg/mL
Sample: Recombinant Human Chondrolectin
Sample: Recombinant Human Chondrolectin
Reviewed Applications
Read 1 review rated 5 using MAB2576 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chondrolectin
Chondrolectin (CHODL) is a 36 kDa type I transmembrane protein containing a C-type lectin carbohydrate recognition domain. Multiple isoforms of human CHODL are generated as a result of alternative promoter usage and/or alternative splicing. Some isoforms are differentially expressed during T cell development. Human and mouse CHODL share 94% amino acid sequence identity in their extracellular region.
Alternate Names
C21orf68, CHODL, MT75, PRED12
Gene Symbol
CHODL
UniProt
Additional Chondrolectin Products
Product Documents for Human Chondrolectin Antibody
Product Specific Notices for Human Chondrolectin Antibody
For research use only
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