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Human Chromogranin A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9098

R&D Systems, part of Bio-Techne
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MAB9098
MAB9098-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 948107

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived human Chromogranin A
Met1-Gly457
Accession # P10645

Specificity

Detects human Chromogranin A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Chromogranin A Antibody

Chromogranin A antibody in Human Parathyroid by Immunohistochemistry (IHC-P).

Chromogranin A in Human Parathyroid.

Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB9098) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in chief cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Chromogranin A Antibody

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human parathyroid

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin A

Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Entrez Gene IDs

1113 (Human); 12652 (Mouse); 24258 (Rat)

Gene Symbol

CHGA

UniProt

Additional Chromogranin A Products

Product Documents for Human Chromogranin A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Chromogranin A Antibody

For research use only

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