Human Claspin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3310
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Claspin
Phe1138-Ser1339
Accession # Q9HAW4
Phe1138-Ser1339
Accession # Q9HAW4
Specificity
Detects human Claspin in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Claspin Antibody
Detection of Human Claspin by Western Blot.
Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Claspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3310) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Claspin at approximately 199 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Claspin in HeLa Human Cell Line.
Claspin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Claspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3310) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Claspin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Claspin
Claspin is a nuclear protein whose expression peaks during S and G2 phases of the cell cycle. Claspin is phosphorylated by ATR in response to DNA damage or replication stress. It functions in cell cycle checkpoint control by regulating the activation of Chk1 and BRCA1. Claspin contains three CKB motifs and two coiled coil regions. Over the range used for immunization, human and mouse Claspin share 72% amino acid sequence identity. DNA replication and DNA damage induce the phosphorylation of Claspin by Chk1-dependent mechanisms.
Alternate Names
CLSPN
Gene Symbol
CLSPN
UniProt
Additional Claspin Products
Product Documents for Human Claspin Antibody
Product Specific Notices for Human Claspin Antibody
For research use only
Loading...
Loading...
Loading...
Loading...