HEK293 human embryonic kidney cell line transfected with (A) human Claudin-11 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-11 Monoclonal Antibody (Catalog # MAB4280R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Claudin-11 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Claudin-11 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-11

Claudin-11 is a 207 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-11 is widely expressed and is involved in epithelial remodeling during spermatogenesis and inner ear mechanotransduction. Claudin-11 is up‑regulated in some cancers. Human Claudin-11 shares 94% aa sequence identity with mouse and rat Claudin-11.

Alternate Names

Claudin11, CLDN11, OSP, OTM

Entrez Gene IDs

5010 (Human); 18417 (Mouse); 84588 (Rat)

Gene Symbol

CLDN11

UniProt

O75508

Additional Claudin-11 Products

Product Documents for Human Claudin-11 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Claudin-11 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Claudin-11 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4280R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Claudin-11 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Claudin-11 Antibody

Detection of Claudin-11 in HEK293 Human Cell Line Transfected with Human Claudin-11 and eGFP by Flow Cytometry.