Human CLP24 and eGFP co-transfectants were stained with Mouse Anti-Human CLP24 Monoclonal Antibody (Catalog # MAB6747) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

CLP24 in NTera‑2 Human Cell Line.

CLP24 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Mouse Anti-Human CLP24 Monoclonal Antibody (Catalog # MAB6747) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CLP24 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human CLP24 and eGFP co-transfectants

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLP24

TMEM204, also known as Claudin-like protein of 24 kDa (CLP24), is a cell surface protein with four transmembrane domains and a C-terminal PDZ motif. TMEM204 localizes to adherens junctions between epithelial and endothelial cells. It is involved in regulating paracellular flux and is upregulated under hypoxic conditions. Human TMEM204 shares 88% aa sequence identity with mouse and rat TMEM204.

Long Name

Claudin-like Protein 24

Alternate Names

C16orf30, TMEM204

Entrez Gene IDs

79652 (Human); 407831 (Mouse); 287129 (Rat)

Gene Symbol

TMEM204

UniProt

Q9BSN7 (Human)

Additional CLP24 Products

Product Documents for Human CLP24 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CLP24 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CLP24 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6747

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CLP24 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CLP24 Antibody

Detection of CLP24 in CLP24 and eGFP Co-Transfected Cells by Flow Cytometry.