HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB9856, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human Coagulation Factor V (Light Chain) Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human Coagulation Factor V (Light Chain) Monoclonal Antibody (Catalog # MAB98561).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor V

Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.

Long Name

Coagulation Factor V

Alternate Names

Cf-5, Cf5, Coagulation Factor 5, F5, Factor V, FVL, PCCF, Proaccelerin, Labile Factor, RPRGL1, THPH2

Entrez Gene IDs

2153 (Human); 14067 (Mouse); 304929 (Rat)

Gene Symbol

UniProt

P12259

Additional Coagulation Factor V Products

Product Documents for Human Coagulation Factor V (Light Chain) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Coagulation Factor V (Light Chain) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Coagulation Factor V (Light Chain) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9856

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Coagulation Factor V Antibodies »

Product Summary for Human Coagulation Factor V (Light Chain) Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Coagulation Factor V (Light Chain) Antibody

Detection of Human Coagulation Factor V in HepG2 Human Cell Line by Flow Cytometry.