Human Collagen XXIII alpha1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4165
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 468642
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Collagen XXIII alpha1
Glu111-Lys540
Accession # NP_775736
Glu111-Lys540
Accession # NP_775736
Specificity
Detects human Collagen XXIII alpha1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50% cross-reactivity with recombinant human Collagen XXV alpha1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Collagen XXIII alpha1 Antibody
Detection of Human Collagen XXIII alpha1 by Western Blot.
Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Collagen XXIII a1 at approximately 75 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Collagen XXIII alpha1 in Human Adenocarcinoma.
Collagen XXIII a1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Collagen XXIII alpha1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human adenocarcinoma
Sample: Immersion fixed paraffin-embedded sections of human adenocarcinoma
Western Blot
2 µg/mL
Sample: NCI‑H460 human large cell lung carcinoma cell line
Sample: NCI‑H460 human large cell lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen XXIII alpha 1
Collagen XXIII alpha1 (COL23A1) is an epithelial cell type 2 transmembrane glycoprotein with multiple collagen like domains in its extracellular region. Intracellular furin mediated cleavage of the 68 kDa molecule generates a 58 kDa shed fragment that forms disulfide linked trimers. An alternately spliced isoform consists of nearly 300 amino acids of the membrane proximal extracellular domain (ECD), flanked by substituted sequences. COL23A1 is upregulated in many transformed cell lines and serves as an indicator for tumor aggressiveness in prostate cancer. Within the ECD, human COL23A1 shares 89% aa sequence identity with mouse and rat COL23A1.
Alternate Names
COL23A1
Gene Symbol
COL23A1
UniProt
Additional Collagen XXIII alpha 1 Products
Product Documents for Human Collagen XXIII alpha1 Antibody
Product Specific Notices for Human Collagen XXIII alpha1 Antibody
For research use only
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