Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with recombinant human IL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross-reactivity with rhIL-2 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Common gamma Chain/IL-2 R gamma Antibody

Detection of Common gamma Chain/IL‑2 R gamma in Human PBMC by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Common gamma Chain/IL-2 R gamma Monoclonal Antibody (Catalog # MAB2842, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody.

Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Human IL-2 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Common gamma Chain/IL-2 R gamma Monoclonal Antibody (Catalog # MAB2842). The ND₅₀ is typically 0.08-0.4 µg/mL.

Applications for Human Common gamma Chain/IL-2 R gamma Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 0.08-0.4 µg/mL in the presence of 200 ng/mL Recombinant Human IL‑2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the common gamma chain ( gamma_c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has been mapped to the IL-2 R gamma gene.

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Noguchi, M. et al. (1993) Science 262:1877.

Long Name

IL-2 Receptor gamma

Alternate Names

CD132, IL-2 R gamma, IL2R gamma, IL2RG

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Gene Symbol

IL2RG

UniProt

P31785

Additional Common gamma Chain/IL-2 R gamma Products

Product Documents

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Common gamma Chain/IL-2 R gamma Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Common gamma Chain/IL-2 R gamma Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2842

Key Product Details

Species Reactivity

Validated:

Cited:

Applications