Human Complement Component C1qC Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3337
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human C1qC
Lys115-Asp245
Accession # P02747
Lys115-Asp245
Accession # P02747
Specificity
Detects human Complement C1qC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) C1qA and rhC1qB is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Complement Component C1qC Antibody
Complement Component C1qC in Human HUVEC.
Complement Component C1qC was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Complement Component C1qC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3337) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Complement Component C1qC Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human Complement Component C1qC
Sample: Recombinant Human Complement Component C1qC
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C1qC
Long Name
Complement Component 1q Subcomponent Subunit C Precursor
Alternate Names
C1qC
Entrez Gene IDs
714 (Human)
Gene Symbol
C1QC
UniProt
Additional Complement Component C1qC Products
Product Documents for Human Complement Component C1qC Antibody
Product Specific Notices for Human Complement Component C1qC Antibody
For research use only
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