Human Complement Component C1r Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1807
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 269104
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Component C1r
Ser18-Asp705
Accession # NP_001724
Ser18-Asp705
Accession # NP_001724
Specificity
Detects human C1r in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) C1s or rhC1q R1 is observed. In Western blots, ths antibody recognizes rhC1r and the A/heavy chain.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Complement Component C1r Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C1r (Catalog # 1807-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C1r (Catalog # 1807-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Complement Component C1r (Catalog # 1807-SE) under non-reducing conditions only
Sample: Recombinant Human Complement Component C1r (Catalog # 1807-SE) under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C1r
The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain.
References
- Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
- Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
- Journet A. and M. Tosi (1986) Biochem. J. 240:783.
Alternate Names
C1r
Entrez Gene IDs
715 (Human)
Gene Symbol
C1R
UniProt
Additional Complement Component C1r Products
Product Documents for Human Complement Component C1r Antibody
Product Specific Notices for Human Complement Component C1r Antibody
For research use only
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