Human Complement Component C1s (Research Grade Sutimlimab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11489
Recombinant Monoclonal Antibody For Research Use Only
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu222
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CD38
Met1-Ile300
Accession # P28907
Met1-Ile300
Accession # P28907
Specificity
Detects human complement component C1s in direct ELISAs.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images
Human Complement Component C1s ELISA Standard Curve
Direct ELISA binding curve demonstrating the recognition of Human Anti-Human Complement Component C1s (Research Grade Sutimlimab Biosimilar) Monoclonal Antibody (Catalog # MAB11489) to Complement Component C1s. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A goat anti-human HRP conjugate was used for detection.Applications
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody for the specific antigen in direct ELISA. Colorimetric detection is performed after addition of a suitable substrate.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C1s
The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑688) of human C1s was expressed (3‑5). The purified protein corresponded to the processed active form, with A and B chains starting at residue Glu16 and Ile438, respectively.
References
- Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
- Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
- Tosi, M. et al. (1987) Biochemistry 26:8516.
- Mackinnon, C.M. et al. (1987) Eur. J. Biochem. 169:547.
- Kusumoto, H. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7307.
Alternate Names
C1S
Entrez Gene IDs
716 (Human)
Gene Symbol
C1S
UniProt
Additional Complement Component C1s Products
Product Specific Notices
For research use only
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