Human Complement Component C9 Antibody

Name: Human Complement Component C9 Antibody
Brand: R&D Systems
SKU: MAB8126
Rating: 2 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB8126

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 887025

View all Complement Component C9 Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Complement Component C9
Met1-Lys559
Accession # P02748

Specificity

Detects human Complement Component C9 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Complement Component C9 Antibody

Detection of Human Complement Component C9 by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Complement Component C9 by Simple Western^TM.

Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Complement Component C9 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Complement Component C9 Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: Human plasma

Western Blot

1 µg/mL
Sample: Human plasma

Reviewed Applications

Read 1 review rated 2 using MAB8126 in the following applications:

complement membrane attack complex (C5b-9) detection (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C9

Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize.

Alternate Names

complement component 9, complement component C9

Entrez Gene IDs

735 (Human); 12279 (Mouse); 117512 (Rat)

Gene Symbol

UniProt

P02748

Additional Complement Component C9 Products

Product Documents for Human Complement Component C9 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Complement Component C9 Antibody

For research use only

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