Human CRTAC1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB52341
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 755339
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRTAC1
Ser28-Cys661
Accession # Q9NQ79
Ser28-Cys661
Accession # Q9NQ79
Specificity
Detects human CRTAC1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CRTAC1 Antibody
Detection of Human CRTAC1 by Western Blot.
Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CRTAC1 Monoclonal Antibody (Catalog # MAB52341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CRTAC1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human CRTAC1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human cartilage tissue
Sample: Human cartilage tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRTAC1
CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.
Long Name
Cartilage Acidic Protein 1
Alternate Names
ASPIC1, CEP68
Gene Symbol
CRTAC1
UniProt
Additional CRTAC1 Products
Product Documents for Human CRTAC1 Antibody
Product Specific Notices for Human CRTAC1 Antibody
For research use only
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