Human CS Citrate Synthase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11583
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1081813
Product Specifications
Immunogen
Synthetic Peptide
Accession # O75390
Accession # O75390
Specificity
Detects a synthetic peptide corresponding to residues near amino acid 400 of human CS protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human CS Citrate Synthase Antibody
Detection of CS Citrate Synthase in Human Liver Cancer.
CS Citrate Synthase was detected in immersion fixed paraffin-embedded sections of human liver cancer using Mouse Anti-Human CS Citrate Synthase Monoclonal Antibody (Catalog # MAB11583) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CS Citrate Synthase Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer
Sample: Immersion fixed paraffin-embedded sections of human liver cancer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CS Citrate Synthase
References
- Wiegand G, Remington SJ. Citrate synthase: structure, control, and mechanism. Annu Rev Biophys Biophys Chem. 1986;15:97-117. doi: 10.1146/annurev.bb.15.060186.000525. PMID: 3013232.
- Gillen JB, Martin BJ, MacInnis MJ, Skelly LE, Tarnopolsky MA, Gibala MJ. Twelve Weeks of Sprint Interval Training Improves Indices of Cardiometabolic Health Similar to Traditional Endurance Training despite a Five-Fold Lower Exercise Volume and Time Commitment. PLoS One. 2016 Apr 26;11(4):e0154075. doi: 10.1371/journal.pone.0154075. PMID: 27115137; PMCID: PMC4846072.
- Zhou L, Ou S, Liang T, Li M, Xiao P, Cheng J, Zhou J, Yuan L. MAEL facilitates metabolic reprogramming and breast cancer progression by promoting the degradation of citrate synthase and fumarate hydratase via chaperone-mediated autophagy. FEBS J. 2023 Jul;290(14):3614-3628. doi: 10.1111/febs.16768. Epub 2023 Mar 12. PMID: 36866961.
Long Name
CS Citrate Synthase
Alternate Names
citrate (Si)-synthase, citrate synthase, mitochondrial, EC 2.3.3.1
Gene Symbol
O75390
UniProt
Additional CS Citrate Synthase Products
Product Documents for Human CS Citrate Synthase Antibody
Product Specific Notices for Human CS Citrate Synthase Antibody
For research use only
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