Detects human CXCL1/2/3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) CXCL2 is observed and no cross-reactivity with recombinant human (rh) VIC, rmVIC, rhCCL3L1, rhCCL4L1, or recombinant canine IL-8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human CXCL1/2/3/GRO Pan Specific Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells treated with PHA, fixed with paraformaldehyde, and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CXCL1/GRO alpha/KC/CINC-1 (Catalog # 275-GR)
Recombinant Human CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 276-GB)
Recombinant Human CXCL3/GRO gamma/CINC-2/DCIP-1 (Catalog # 277-GG)
under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/2/3/GRO

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.

Alternate Names

FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1

UniProt

P19876

Additional CXCL1/2/3/GRO Products

Product Documents for Human CXCL1/2/3/GRO Pan Specific Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CXCL1/2/3/GRO Pan Specific Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CXCL1/2/3/GRO Pan Specific Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2761

Key Product Details

Species Reactivity

Validated:

Cited:

Applications