Human CXCL11/I-TAC Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB672
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
Bioassay, ELISA Development, Flow Cytometry, Immunocytochemistry, Immunodepletion, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 87328
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL11/I-TAC
Phe22-Phe94
Accession # O14625
Phe22-Phe94
Accession # O14625
Specificity
Detects human CXCL11/I-TAC in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL5, 7, 8, 9, or 10. In Western blots, this antibody shows less than 1% cross-reactivity with rhCXCL1, 2, 3, 5, 6, 7, IL-8/CXCL8, 9, 10, 13, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, rmCXCL1, 2, 6, 9, 10, CXCL12/SDF-1 alpha, rmCXCL13, rrCXCL1, CXCL3/CINC-2 alpha, CXCL3/CINC-2 beta, or rpIL-8/CXCL8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CXCL11/I-TAC Antibody
Chemotaxis Induced by CXCL11/I-TAC and Neutralization by Human CXCL11/I-TAC Antibody.
Recombinant Human CXCL11/I-TAC (672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (15 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB672). The ND50 is typically 0.0400-0.480 µg/mL.Applications for Human CXCL11/I-TAC Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CXCL11/I-TAC (Catalog # 672-IT)
Sample: Recombinant Human CXCL11/I-TAC (Catalog # 672-IT)
Neutralization
Measured by its ability to neutralize CXCL11/I-TAC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.0400-0.480 μg/mL in the presence of 15 ng/mL Recombinant Human CXCL11/I-TAC.
Human CXCL11/I-TAC Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL11/I-TAC
CXCL11, also known as I-TAC, SCYB9B, H174 and beta-R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen, and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3 but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.
References
- Cole, K. et al. (1998) J. Exp. Med. 187:2009.
- Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
- Lou, Y. et al. (1998) J. Neurovirol. 4:575.
Alternate Names
beta-R1, H174, I-TAC, ITAC, SCYB9B
Gene Symbol
CXCL11
UniProt
Additional CXCL11/I-TAC Products
Product Documents for Human CXCL11/I-TAC Antibody
Product Specific Notices for Human CXCL11/I-TAC Antibody
For research use only
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