Human CXCL6/GCP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB333
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 60910
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL6/GCP-2
Val40-Asn114
Accession # P80162
Val40-Asn114
Accession # P80162
Specificity
Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha, rhGRO beta, rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha, and rhSDF-1 beta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CXCL6/GCP-2 Antibody
Chemotaxis Induced by CXCL6/GCP‑2 and Neutral-ization by Human CXCL6/ GCP‑2 Antibody.
Recombinant Human CXCL6/ GCP-2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP-2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP-2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 µg/mL.Applications for Human CXCL6/GCP-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC)
Sample: Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC)
Neutralization
Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 8-30 μg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2.
Human CXCL6/GCP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB333 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL6/GCP-2
Additional CXCL6/GCP-2 Products
Product Documents for Human CXCL6/GCP-2 Antibody
Product Specific Notices for Human CXCL6/GCP-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...