Human Cyclin A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7046
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 722407
Product Specifications
Immunogen
E. coli-derived recombinant human Cyclin A1
Lys51-Arg220
Accession # P78396
Lys51-Arg220
Accession # P78396
Specificity
Detects human Cyclin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no
cross-reactivity with recombinant human Cyclin A2, B1, B2, C, D1, D2, D3, E1,
E2, or recombinant mouse Cyclin A1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Cyclin A1 Antibody
Detection of Human Cyclin A1 by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin A1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cyclin A1 in Human Testis.
Cyclin A1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cyclin A1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis
Sample: Immersion fixed paraffin-embedded sections of human testis
Western Blot
1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line, MCF‑7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue
Sample: THP‑1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line, MCF‑7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin A1
Alternate Names
CCNA1
Gene Symbol
CCNA1
UniProt
Additional Cyclin A1 Products
Product Documents for Human Cyclin A1 Antibody
Product Specific Notices for Human Cyclin A1 Antibody
For research use only
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