Human/Cynomolgus Monkey IL-23R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB106242

R&D Systems, part of Bio-Techne
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MAB106242-100
MAB106242-SP
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Key Product Details

Species Reactivity

Human, Cynomolgus Monkey

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1032638

View all IL-23R Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived cynomolgus monkey IL-23R
Gly24-Asp353
Accession # XP_005543141.1

Specificity

Detects cynomolgus monkey IL-23R in direct ELISAs and human IL-23R in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Cynomolgus Monkey IL-23R Antibody

Detection of Human IL-23R by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Cynomolgus Monkey IL-23R Monoclonal Antibody (Catalog # MAB106242) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018) . A specific band was detected for IL-23R at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human/Cynomolgus Monkey IL-23R Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta 1) and the IL-23-specific receptor subunit (IL-23R) (3). Human IL-23R cDNA encodes a 629 amino acids (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12R beta 2. IL-23R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif (6). The cytoplasmic region of IL-23R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23R is located on human chromosome 1 within 150 kb of IL-12R beta 2. Based on quantitative real-time PCR, human IL-23R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5 (2). The Cynomolgus IL-23R shares 96%, 71% and 77% amino acid sequence identity to Human, mouse, and rat IL-23R, respectively.

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and Frucht, D.M. (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
  6. Schroder, J. et al. (2015) J. Biol. Chem. 290:359.

Long Name

Interleukin 23 Receptor

Alternate Names

IL-23 R, IL23R

Entrez Gene IDs

149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)

Gene Symbol

IL23R

UniProt

XP_005543141.1

Additional IL-23R Products

Product Documents for Human/Cynomolgus Monkey IL-23R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Cynomolgus Monkey IL-23R Antibody

For research use only

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