Human Cystatin B Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1408
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cystatin B (R&D Systems, Catalog # 1408-PI)
Met2-Phe98
Accession # P04080
Met2-Phe98
Accession # P04080
Specificity
Detects human Cystatin B in Western blots. In this format, less than 2% cross-reactivity with recombinant mouse Cystatin B, recombinant human (rh) Cystatin A, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin S, rhCystatin SA, and rhCystatin SN is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Cystatin B Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Cystatin B (Catalog # 1408-PI)
Sample: Recombinant Human Cystatin B (Catalog # 1408-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin B
Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
- Pennacchio, L.A. et al. (1996) Science 271:1731.
- Zhang, R. et al. (2003) Glia 42:194.
- Zerovnik, E. et al. (2002) Biochim. Biophys. Acta 1594:1.
Alternate Names
CSTB, Stefin B
Gene Symbol
CSTB
UniProt
Additional Cystatin B Products
Product Documents for Human Cystatin B Biotinylated Antibody
Product Specific Notices for Human Cystatin B Biotinylated Antibody
For research use only
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