Human Cystatin C Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11962
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 197820
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin C
Ser27-Ala146
Accession # P01034
Ser27-Ala146
Accession # P01034
Specificity
Detects human Cystatin C in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human Cystatins A, B, D, E/M, F, S, SA, SN, X/Z/P, or recombinant mouse Cystatin C is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Cystatin C Antibody
Application
Recommended Usage
Human Cystatin C Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB11962 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin C
Cystatin C is a member of family 2 of the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).
References
- Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
- Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
- Laterza, O.F. et al. (2002) Clin. Chem. 48:699.
Alternate Names
ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Gene Symbol
CST3
UniProt
Additional Cystatin C Products
Product Documents for Human Cystatin C Antibody
Product Specific Notices for Human Cystatin C Antibody
For research use only
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