Human Cystatin D Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1202
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin D
Thr29-Val142
Accession # CAA49838
Thr29-Val142
Accession # CAA49838
Specificity
Detects human Cystatin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Cystatin C, rhCystatin SN, and rhCystatin S is observed and less than 1% cross-reactivity with rhCystatin E/M and rhFetuin A is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Cystatin D Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Cystatin D (Catalog # 1202-PI)
Sample: Recombinant Human Cystatin D (Catalog # 1202-PI)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin D
Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).
References
- Freije, J.P. et al. (1993) J. Biol. Chem. 268:15737.
- Balbin, M. et al. (1994) J. Biol. Chem. 269:23156.
- Collins, A.R. and A. Grubb (1998) Oral Microbiol. Immunol. 13:59.
Alternate Names
CST5, CSTD
Entrez Gene IDs
1473 (Human)
Gene Symbol
CST5
UniProt
Additional Cystatin D Products
Product Documents for Human Cystatin D Antibody
Product Specific Notices for Human Cystatin D Antibody
For research use only
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