Human Cystatin S Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1296
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
ELISA Development, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 201805
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin S
Ser21-Ala141
Accession # P01036
Ser21-Ala141
Accession # P01036
Specificity
Detects human Cystatin S in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin SA is observed and no cross-reactivity with rhCystatin D, E, SN, rhFetuin A, rhFetuin B, or rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed. In Western blots, approximately 15% cross‑reactivity with rhCystatins SA and SN (under reducing conditions only) is observed and no cross‑reactivity with rhCystatin A, B, C, D, E/M, rhFetuin A, rhFetuin B, or rhHPRG is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Cystatin S Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin S (Catalog # 1296-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin S (Catalog # 1296-PI), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Cystatin S (Catalog # 1296-PI)
Sample: Recombinant Human Cystatin S (Catalog # 1296-PI)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin S
Cystatin S is a member of family 2 of the Cystatin superfamily (1). Like Cystatins SA and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Unlike Cystatins SA and SN, Cystatin S is not a potent inhibitor of cysteine proteases such as papain and some cathepsins (3). Cystatin S is partially phosphorylated at Ser21 and Ser23 in saliva (4). The functions of Cystatin S are largely unknown. However, it is able to bind more calcium and bind more rapidly to carbonated apatite than cystatins SA or SN, indicating that Cystatin S may be involved in the mineral balance of the tooth (3).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Baron, A.C. et al. (1999) Oral. Dis. 5:344.
- Baron, A. et al. (1999) Oral Dis. 5:234.
- Isemura, S. et al. (1991) J. Biochem. 110:648.
Alternate Names
CST4
Entrez Gene IDs
1472 (Human)
Gene Symbol
CST4
UniProt
Additional Cystatin S Products
Product Documents for Human Cystatin S Antibody
Product Specific Notices for Human Cystatin S Antibody
For research use only
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