Human Cytohesin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4807
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cytohesin-1
Met1-Tyr102
Accession # Q15438
Met1-Tyr102
Accession # Q15438
Specificity
Detects endogenous human Cytohesin-1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Cytohesin-1 Antibody
Detection of Human Cytohesin-1 by Western Blot.
Western blot shows lysates of NK-92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Cytohesin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cytohesin-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Cytohesin-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NK-92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line
Sample: NK-92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytohesin-1
Cytohesin-1, previously called B2-1 or PSCD1 (pleckstrin homology, Sec7 and coiled-coil domain-containing protein 1), is a 398 amino acid (aa), 47 kDa member of the cytohesin family of guanine nucleotide exchange factors. The Sec7 domain (aa 62‑244) binds the integrin beta 2 subunit cytoplasmic domain, activating integrins via inside-out signaling, thus implicated in chemotaxis, adhesion, and cell spreading. The region of human Cytohesin-1 used as an immunogen shares 97% and 96% aa identity with mouse and rat Cytohesin-1, and 67% and 69% aa identity with human cytohesins 2 and 3, respectively. Although ubiquitously expressed, Cytohesin-1 is primarily found in immune system cells, and was cloned from a natural killer (NK) cell minus T helper cell line Jurkat library.
Alternate Names
B2-1, CYTH1, Cytohesin1, PSCD1, SEC7
Gene Symbol
CYTH1
UniProt
Additional Cytohesin-1 Products
Product Documents for Human Cytohesin-1 Antibody
Product Specific Notices for Human Cytohesin-1 Antibody
For research use only
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