Human Cytokeratin 8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3165
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # LP3K
Product Specifications
Immunogen
SV-40 transformed human neonatal keratinocyte extract
Specificity
Detects human Cytokeratin 8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Cytokeratin 8 Antibody
Cytokeratin 8 in MCF‑7 Human Cell Line.
Cytokeratin 8 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cytokeratin 8 in Human Colon Cancer Tissue.
Cytokeratin 8 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cytokeratin 8 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed LS180 human colorectal adenocarcinoma cell line and immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed LS180 human colorectal adenocarcinoma cell line and immersion fixed MCF-7 human breast cancer cell line
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Western Blot
Lane, E.B. et al. (1985) Ann. NY Acad. Sci. 455:241. This application was not tested by R&D Systems.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 7 reviews rated 4.1 using MAB3165 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 8
Alternate Names
CARD2, CK8, EndoA, KRT8
Gene Symbol
KRT8
Additional Cytokeratin 8 Products
Product Documents for Human Cytokeratin 8 Antibody
Product Specific Notices for Human Cytokeratin 8 Antibody
For research use only
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